Journal: iScience

Article Title: Exercise is associated with attenuated aging-related osteoporosis through TMAO alpha Klotho inflammasome signaling

doi: 10.1016/j.isci.2026.117170

Figure Lengend Snippet: Exercise suppresses TMAO-induced bone resorption and systemic inflammation in aged rats (A and B) (A) Femoral marrow TMAO (ng/g) and (B) plasma TMAO (nmol/L) quantified by ultra‑high‑performance liquid chromatography‑mass spectrometry (UHPLC-MS)/MS. Exercise lowered TMAO relative to the model (E < M), whereas TMAO loading increased TMAO (T > M) and was partially rescued by exercise (T + E < T). (C) Serum CTX (pg/mL) was elevated by TMAO and reduced by exercise, consistent with attenuation of bone resorption. (D) Serum PINP (ng/mL) showed the opposite pattern—elevated with exercise, reduced by TMAO, and restored in T + E. (E) Volcano plot of differentially expressed circulating cytokines between model (M) and TMAO (T) groups; significantly altered proteins are highlighted. (F) Heatmap of selected cytokines across individual animals (M-1–M-3, T-1–T-3) showing higher IL-6, CINC-3, GM-CSF, and related mediators in T. (G) Volcano plot comparing T vs. T + E demonstrates cytokine shifts with exercise. (H) Heatmap illustrates a reduction in the pro-inflammatory cytokine IL-6 and an increase in the anti-inflammatory cytokine IL-10 in T + E relative to T. Bars indicate mean ± SD; dots are individual animals ( n per group in ). n = 3 rats per group for (A)–(D). Statistical comparisons for (A)–(D) were performed using one-way ANOVA followed by Tukey’s multiple-comparisons post hoc test. For cytokine antibody array analyses in (E)–(H), n = 3 rats per group; normalized signal intensities were used for two-group screening of differentially expressed cytokines, and heatmaps display normalized relative intensities. ∗ p < 0.05; ns, not significant. Group labels: C, control; M, model; E, exercise; T, TMAO; T + E, TMAO plus exercise.

Article Snippet: RayBiotech Rat Cytokine Antibody Array , RayBiotech , Cat# AAR-CYT-1.

Techniques: Clinical Proteomics, Tandem Mass Spectroscopy, Ab Array, Control

Journal: iScience

Article Title: Exercise is associated with attenuated aging-related osteoporosis through TMAO alpha Klotho inflammasome signaling

doi: 10.1016/j.isci.2026.117170

Figure Lengend Snippet:

Article Snippet: RayBiotech Rat Cytokine Antibody Array , RayBiotech , Cat# AAR-CYT-1.

Techniques: Immunofluorescence, Immunoprecipitation, Clinical Proteomics, Recombinant, Enzyme-linked Immunosorbent Assay, Staining, CCK-8 Assay, Ab Array, Control, Negative Control, Sequencing, Plasmid Preparation, Clone Assay, Expressing, Software, Micro-CT, Real-time Polymerase Chain Reaction, Fluorescence, Microscopy

Journal: International journal of molecular sciences

Article Title: 2'-Hydroxycinnamaldehyde, a Natural Product from Cinnamon, Alleviates Ischemia/Reperfusion-Induced Microvascular Dysfunction and Oxidative Damage in Rats by Upregulating Cytosolic BAG3 and Nrf2/HO-1.

doi: 10.3390/ijms252312962

Figure Lengend Snippet: Figure 10. Effect of HCA preconditioning on cardiac I/R injury-induced expression of multiple cytokines in the three groups determined using cytokine array analysis. (A) Original cytokine expression. (B) Matching dot plot. The expression levels of TCK-1 (C), IL-1 R6 (D), ICAM-1 (E), MMP-8 (F), agrin (G), TNF-α (H), IL-2 (I), IL-4 (J), IL-10 (K), LIX (L), CINC-1 (M), and prolactin (N) are significantly elevated in I/R vs. sham group, whereas they are significantly decreased in HCA+I/R vs. I/R group. All data are expressed as mean ± SEM (n = 4). * indicates significant differences vs. sham group (** p < 0.01; *** p < 0.001; **** p < 0.0001). # indicates the significant differences vs. I/R group (# p < 0.05; ## p < 0.01; ### p < 0.001; #### p < 0.0001).

Article Snippet: The expression levels of multiple cytokines were determined using a RayBio®rat cytokine Antibody Array C1 (RayBiotech, Inc., Norcross, GA, USA) according to the manufacturer’s instructions.

Techniques: Expressing